Protein_Domain
Xyla

Part:BBa_K1362441:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

Xylanase (cds only)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 405
    Illegal SapI.rc site found at 459


Design Notes

This basic part is an element of BBa_K1362020, BBa_K1362021, BBa_K1362022 and BBa_K1362023. The start codon ATG is not included, because this sequence is an element of fusion proteins.

Source

The xylanase gene was extracted from B. subtilis genome via PCR.

References